Accuri Cytometers

Apoptosis

The study of programmed cell death, apoptosis, has broad application in the fields of cell biology, cancer research and developmental biology. A wide variety of products designed to monitor the progressive stages of apoptosis by flow cytometry have been developed over the past ten years. Often, the limiting factors for investigators wishing to develop apoptosis studies are an understanding of reagent compatibility and access to flow cytometry for analysis.

The BD Accuri C6 with BD CFlow Plus Software provides many advantages for apoptosis studies:

  • Absolute counts - Cell concentration can be calculated directly from BD CFlow software statistics tables without the addition of counting beads.
  • Six detection parameters - The dual laser (blue and red) excitation, 4-color detection system allows flexibility in reagent choice.
  • Post collection data analysis - The unique 7-log dynamic range and pre-optimized detector performance minimizes the potential for data loss due to incorrect instrument set up.
  • Digital signal processing - Data signal processing allows post-data collection, fluorescence compensation and gating decision adjustments.
  • Well-established, cost-effective apoptosis kits - BD Biosciences offers a range of kits ideally suited for the BD Accuri C6 including kits that analyze plasma membrane alterations, mitochondrial membrane changes, caspase activation and DNA fragmentation.

    • Resources and Kits: