Accuri Cytometers

Cell Cycle and DNA Analysis

Biological experiments from the plant kingdom illustrate several key performance features of the Accuri C6. Plant nuclear DNA content (ploidy) varies over extreme ranges and quantitative measurement of the characteristic “C-value” is achieved by detecting propidium iodide fluorescence using flow cytometry. Nuclear DNA measurements are often hampered by excessive cellular and sub-cellular debris and autofluorescence from other prevalent cellular components, such as chloroplasts.

With over 7 decades of digital signal linearity, the Accuri C6 Flow Cytometer is the first flow cytometer to cover the full biological range of flowering plant genome sizes from 0.32 to 80.9 picograms in a single run. With the high degree of reproducibility, the requirement for using internal standards may be lessened, over time.

Additional Resources:

  • Examples of Cell Cycle and DNA Analysis studies on the Accuri C6 (0.19 MB)
  • Simultaneous Analysis of GFP-Transgene Expression and Plant Cell Ploidy (0.12 MB)
  • Using the Accuri C6 Flow Cytometer for Rapid and Accurate Analysis of the Nuclear DNA Contents of Flowering Plants
  • Refer to Technical Information and Kits for more resources