C6 Flow Cytometer® Instrument
The Accuri C6 Flow Cytometer, CFlow® Software and Workstation PC supply all the capabilities of a full-featured flow cytometer at a fraction of the price of the market leading systems. The C6 system includes blue and red lasers, four color detectors and both forward and side scatter detectors plus software that is so intuitive that you will typically be up and running within an hour of receiving your Accuri system.
Click for a larger image of the Accuri C6 Flow Cytometer.
A simple design for complex research.
Our engineers had a daunting task-build a cytometer that is compact, simple to operate, easy to afford, and matches the performance of the best selling analytical flow cytometers. We started by listening to scientists like you and then designing a flow cytometer from the ground up incorporating your needs. We worked hard to provide the performance for most experiments, while making the C6 easier to use, simple to maintain, and affordable for everyone.
Accuri's innovations in fluidics, optics, electronics and software, coupled with state-of-the-art components, result in a cytometer that is powerful, robust, compact and affordable. We also developed an intuitive user interface that can be mastered in minutes. And we've done all of this so you can focus on one thing: advancing your science, in your lab.
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Accuri C6 Flow Cytometer Specifications
Accuri's C6 Flow Cytometer is a high performance analytical cytometer that matches the functionality of the best selling analytical flow cytometers (see comparison link to table below). Features of the C6 include:
1. Two laser excitation:
- 488nm diode laser: excites FITC, GFP, PE, PerCP, PerCp-Cy 5.5 & PI
- 640nm diode laser: excites APC
2. Two scattered light detectors:
- Forward scatter (FSC)
- Side scatter (SSC)
3. Four fluorescent emission detectors:
- FL1 530+/-15nm (FITC/GFP)
- FL2 585+/-20nm (PE/PI)
- FL3 >670nm (PerCP-Cy5.5,PE-Cy5, PE-Cy7)
- FL4 675+/-12.5nm (APC)
Advanced Features
On this page:
- 6 Decade dynamic range
- Optical bench needs no alignment
- Fluidics control
- Absolute counts
- Maintenance made simple
- Works with current protocols and reagents
- FCS 3.0 compatible file format
- Compact size
- Low power usage
6 Decade dynamic range: The totally digital C6 Flow Cytometer has state-of-the-art electronics that make it possible to simultaneously collect 16 million channels of digital data. This unparalleled dynamic range obviates the need for adjustable gain settings on the detectors. Data is collected and always remains in the data file even when you use the CFlow Zoom function to view specified channels. And eliminating the need to set gain or voltage reduces the consumption of valuable sample and sheath during set-up.
Optical bench needs no alignment: The C6 optical bench is compact and employs components that have evolved to be smaller, more robust, and less expensive. This smaller optical bench needs no alignment so the C6 is ready to use out of the box.
Fluidics control: The C6 uses a unique low-pressure pumping system to drives fluidics as opposed to more expensive pressurized systems. An industry-standard, sheath-focused core facilitates high performance while enabling event rates of up to 10,000 events/second and sample concentration over 5 x 107 cells/ml. The user control the speed of the sample uptake and can chose the optimal core size for their sample.
Absolute counts: Because the C6 Flow Cytometer fluidics system meters the sample fluid uptake, it is simple to obtain absolute counts, or sample concentration per microliter without the need to add beads to samples. In fact the CFlow Plus software will automatically calculate the events per microliter for every run!
Maintenance made simple: The C6 automatically runs Decontamination fluids through the fluidics lines and then replaces it with sheath upon instrument shutdown. Sheath fluid will remain in the fluidics lines until the instrument is turned on again. A cleaning cycle can also be initiated with a mouse click.
Works with currernt protocols and reagents: Accuri developed the C6 to use the same reagents you currently use on the market-leading cytometers. Since the C6 fluidics system is non-pressurized, any brand of sample tube that is 12 x 75 mm or smaller can be used. This includes microfuge tubes and tubes made of polystyrene and polypropylene.
FCS 3.0 compatible file format: The C6 allows you to export your CFlow files in FCS 3.0 format for seamless importation of your data into most third party flow cytometry software packages including FCS Express and FlowJo. And you can drag and drop plots into MS Office applications, copy and paste statistics into spreadsheets, making data reporting easy.
Compact size: The C6 Flow Cytometer System is designed to fit easily into any lab, even into a laminar flow hood. The bench top cytometer instrument is approximately 2 cubic feet and at less than 30 pounds it is easy to reposition as needs change. No other flow cytometry system packs so much analytical power in so small a package.
Low power usage: The C6 Flow Cytometer operates on a low power, universal power supply (100-240V, 50-60Hz). There are no special electrical requirements for installation, making it easy to place the C6 Flow Cytometer wherever it is needed.
Comparison of Key Specifications of Selected Competitors
None of the competitive products can approach Accuri's combination of price and performance. View the comparison chart for a comparison of Accuri and competitive products. View comparison chart (PDF)
C6 Flow Cytometer: Detailed Specifications
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| Excitation | : | 488nm (rated at 20,000hr life) 640nm (rated at 20,000hr life) |
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| Laser profile | : | 15 x 75 microns |
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| Scatter detection | : | Forward (0 degrees, +/-15) Side (90 degrees, +/-15) |
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| Emission detection | : | 4 colors, user swappable optical filters Standard set installed:
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| Optical alignment | : | Fixed alignment, no maintenance required |
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| Flow cell | : | 200 micron ID quartz capillary |
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| Minimum particle size | : | 1 µm |
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| Minimum sample size | : | 30 µl |
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| Nominal flow rate - variable | : | 10 to 100 µl/min ; CFlow Plus only |
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| Fixed Flow Rates & Core Sizes | : | Slow - (Flow = 14uL/min ; Core = 10 um)
Medium - (Flow = 35uL/mim ; Core = 16 um) Fast - (Flow = 66 uL/min ; Core = 22 um) |
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| Recommended Sheath Fluid | : | 0.2 um filtered DI water |
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| Core diameter | : | User selectable from 5 to 40 microns, CFlow Plus Only |
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| Florescence sensitivity | : | <750 MESF FITC |
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| Scatter resolution | : | Resolves human peripheral blood erythrocytes, lymphocytes, monocytes & granulocytes |
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| Fluorescence linearity | : | 2+/-0.05% for chick embryonic nuclei (CEN) |
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| Fluorescence precision | : | <3% CV for CEN |
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| Speed | : | 10,000 events/second maximum |
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| Absolute counts | : | Yes; CFlow Plus only |
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| Warm-up time | : | Less than 5 minutes |
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| Power | : | 70 Watts maximum, 100-240 VAC, 50/60 Hz |
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| Heat output | : | 240 Btu/hour maximum output |
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| Operating ranges | : | 15-30°C; <80% relative humidity |
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| Instrument size | : | 11"H x 14.3"W x 16.5"D (27.9 x 36.3 x 41.9 cm) Foot print with fluid tanks 17.3"W x 16.3"D |
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| Weight | : | 30 lbs |
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| Fluid tank capacity | : | 1L Sheath Fluid, 1L Waste 250mL Cleaner, 250mL Decontamination |
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| Signal processing | : | 24-bit A/D conversion |
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| Computer | : | 17" flat-panel monitor 2GB memory |
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| Computer interface | : | USB 2.0 |
View the Accuri Price List.
