Selectable Lasers Module

The detectors and lasers of the standard BD Accuri C6 flow cytometer operate in a pre-defined configuration such that detectors FL1, FL2 and FL3 read blue laser-excited fluorescence emissions while detector FL4 reads red laser-excited emissions. This configuration is referred to as “3-blue 1-red”.

Installation of the optional Selectable Lasers Module (Cat. No. 653126) on the BD Accuri C6 allows operation of the system in two other configurations and significantly expands the fluorochrome combinations that can be analyzed:

• 2-blue 2-red: FL1 and FL2 read blue laser-excited emissions; FL3 and FL4 read red laser-excited emissions.
• 4-blue: All 4 detectors read blue laser-excited emissions.

The Selectable Lasers Upgrade requires installation of BD CFlow Plus or the BD CSampler.

Components Supplied

• Selectable Lasers Activation Key
• Three optical filters: 780/60, 610/20, 630/30

Example Fluorochrome Combinations for the Selectable Lasers Module

2-blue 2-red configuration FITC, PE, APC-Cy7, APC Figure 1: Human MNC stained with CD3-APC-Cy7, CD4-APC (left) and CD45RA-FITC, CD45RO-PE (right), analyzed using 2-blue 2-red laser configuration.

4-blue configuration FITC, PE, PE-Cy5, PE-Cy7 FITC , PE, PI, PE-Cy7 and one of the following: PE-Cy5/PerCP/PerCP-Cy5.5 FITC, PE, PE-TexasRed, PECy5 or PE-Cy7 Figure 2: Lymphocyte-gated plot of CD3-PECy5 and CD4-PECy7, analyzed using 4-blue laser configuration.